Structure and Interactions of Fully Hydrated Dioleoylphosphatidylcholine Bilayers
نویسندگان
چکیده
منابع مشابه
Structure and interactions of fully hydrated dioleoylphosphatidylcholine bilayers.
This study focuses on dioleoylphosphatidylcholine (DOPC) bilayers near full hydration. Volumetric data and high-resolution synchrotron x-ray data are used in a method that compares DOPC with well determined gel phase dipalmitoylphosphatidylcholine (DPPC). The key structural quantity obtained is fully hydrated area/lipid A0 = 72.2 +/- 1.1 A2 at 30 degrees C, from which other quantities such as t...
متن کاملStructure Determination of Fully Hydrated L . Phase Dipalmitoylphosphatidylcholine Bilayers
Bilayer form factors obtained from x-ray scattering data taken with high instrumental resolution are reported for multilamellar vesicles of La, phase lipid bilayers of dipalmitoylphosphatidylcholine at 500C under varying osmotic pressure. Artifacts in the magnitudes of the form factors due to liquid crystalline fluctuations have been eliminated by using modified Caille theory. The Caille fluctu...
متن کاملTemperature dependence of structure, bending rigidity, and bilayer interactions of dioleoylphosphatidylcholine bilayers.
X-ray diffuse scattering was measured from oriented stacks and unilamellar vesicles of dioleoylphosphatidylcholine lipid bilayers to obtain the temperature dependence of the structure and of the material properties. The area/molecule, A, was 75.5 A(2) at 45 degrees C, 72.4 A(2) at 30 degrees C, and 69.1 A(2) at 15 degrees C, which gives the area expansivity alpha(A) = 0.0029/deg at 30 degrees C...
متن کاملCloser look at structure of fully hydrated fluid phase DPPC bilayers.
X-ray data are presented for the benchmark dipalmitoylphosphatidylcholine lipid bilayer in the most biologically relevant state in which the bilayers are fully hydrated and in the fluid (liquid-crystalline) phase. Form factors F(q(z)) are obtained from a combination of two sample preparations, oriented stacks of bilayers for q(z) extending to 0.85 A(-1) and unilamellar vesicles for smaller q(z)...
متن کاملSolubilization of Dioleoylphosphatidylcholine Bilayers in Liposomes
Mixtures of iV-dodecyl-Ar,#-dimetriylamine A-oxide (DDAO) and 1,2dioleoylphosphatidyl choline (DOPC) in chloroform/methanol were evaporated, dried and hydrated in excess 2 H 2 0 . Aqueous dispersions thus prepared were ex truded through polycarbonate filter with pores of diameter 500Á These samples were studied using small-angle neutron scattering. DDAO destabilizes the bilayer m unilamellar l...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1998
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(98)77580-0